Composite

Part:BBa_K3989025:Design

Designed by: Song-Yuan Zhang   Group: iGEM21_UZurich   (2021-10-01)


EsaR PesaS GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2049
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2050
    Illegal PstI site found at 2064
    Illegal NotI site found at 7
    Illegal NotI site found at 2057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1009
    Illegal BamHI site found at 1306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2050
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2050
    Illegal PstI site found at 2064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2036
    Illegal BsaI.rc site found at 1313


Design Notes

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Source

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References